Hotspot

trnH-GUG-psbA

LSC · 69-495 · 427 bp

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Pi 0.0124

MarkerSeek score 60.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 427
variable_sites: 17
pi: 0.0124
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	458-503 bp	1.0	85.1
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.2	57.9	459-504 bp	1.0	84.9
trnH-GUG-psbA_p3	AATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.7	57.9	451-496 bp	1.0	83.5
trnH-GUG-psbA_p4	CTGCCTTAATCCACTTGGCT	TAGACCTAGCTGCGGTTGAA	50.0	50.0	57.9	58.4	451-496 bp	1.0	82.1
trnH-GUG-psbA_p5	CGCATGGTGGATTCACAATC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.5	57.9	480-525 bp	0.875	76.1