Hotspot

ycf4

LSC · 59409-59963 · 555 bp

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Pi 0.0018

MarkerSeek score 58.80

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 555
variable_sites: 1
pi: 0.0018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	TGCGAGTTACTTCGGAAACA	CGTTTTTACCCTCCTGCTGA	45.0	50.0	57.5	57.8	687 bp	1.0	74.6
ycf4_p2	TGCGAGTTACTTCGGAAACA	AGCATTGATTTCTCGGCTCA	45.0	45.0	57.5	57.6	664 bp	1.0	72.8
ycf4_p3	TGATGCGAGTTACTTCGGAA	CGTTTTTACCCTCCTGCTGA	45.0	50.0	57.0	57.8	690 bp	1.0	70.5
ycf4_p4	TTGATGCGAGTTACTTCGGA	CGTTTTTACCCTCCTGCTGA	45.0	50.0	57.0	57.8	691 bp	1.0	70.5
ycf4_p5	ATGCGAGTTACTTCGGAAACA	CGTTTTTACCCTCCTGCTGA	42.9	50.0	58.0	57.8	688 bp	1.0	70.4