Hotspot

ycf2

IRb · 86018-92890 · 6873 bp

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Pi 0

MarkerSeek score 19.10

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRb
length_bp: 6873
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 19.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	GGATGCTTAGCGGGTCATAG	ACATTCCGTACCTTGCGAAA	55.0	45.0	57.9	57.8	1229 bp	1.0	77.9
ycf2_p2	TGCTTAGCGGGTCATAGTGA	ACATTCCGTACCTTGCGAAA	50.0	45.0	58.5	57.8	1226 bp	1.0	74.5
ycf2_p3	ATGCTTAGCGGGTCATAGTG	ACATTCCGTACCTTGCGAAA	50.0	45.0	57.5	57.8	1227 bp	1.0	74.4
ycf2_p4	TGGATGCTTAGCGGGTCATA	ACATTCCGTACCTTGCGAAA	50.0	45.0	58.6	57.8	1230 bp	1.0	74.0
ycf2_p5	GCTTAGCGGGTCATAGTGAA	ACATTCCGTACCTTGCGAAA	50.0	45.0	57.4	57.8	1225 bp	1.0	73.8