Hotspot

ccsA

SSC · 122010-122978 · 969 bp

Back to Arctium

Pi 0.00103

MarkerSeek score 59.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 1
pi: 0.00103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AGAACCGCGTGAATCACTAC	TCTTAATTAATCTAGGGGATTCC	50.0	34.8	58.0	52.8	1126 bp	1.0	40.1
ccsA_p2	AGAACCGCGTGAATCACTAC	TTCTTAATTAATCTAGGGGATTCC	50.0	33.3	58.0	53.6	1127 bp	1.0	40.1
ccsA_p3	TGATTCACATGGTTCTCGCC	TCTTAATTAATCTAGGGGATTCC	50.0	34.8	58.3	52.8	1100 bp	1.0	40.1
ccsA_p4	GAGAACCGCGTGAATCACTA	TCTTAATTAATCTAGGGGATTCC	50.0	34.8	57.7	52.8	1127 bp	1.0	40.1
ccsA_p5	GAACCGCGTGAATCACTACA	TCTTAATTAATCTAGGGGATTCC	50.0	34.8	58.3	52.8	1125 bp	1.0	40.1