Hotspot

ycf2-trnL-CAA

IRb · 92891-93298 · 408 bp

Back to Arctium

Pi 0.00245

MarkerSeek score 56.20

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: IRb
length_bp: 408
variable_sites: 1
pi: 0.00245
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	GCTTTTCCCGGATGAAATGC	AAATGGTAGACACGCGAGAC	50.0	50.0	58.1	58.0	562 bp	1.0	79.5
ycf2-trnL-CAA_p2	GCTTTTCCCGGATGAAATGC	TGAAATGGTAGACACGCGAG	50.0	50.0	58.1	58.0	564 bp	1.0	79.5
ycf2-trnL-CAA_p3	GCTTTTCCCGGATGAAATGC	GCCTTGGTGGTGAAATGGTA	50.0	50.0	58.1	58.1	574 bp	1.0	78.9
ycf2-trnL-CAA_p4	ATGGCTTTTCCCGGATGAAA	AAATGGTAGACACGCGAGAC	45.0	50.0	57.8	58.0	565 bp	1.0	78.2
ycf2-trnL-CAA_p5	TGGCTTTTCCCGGATGAAAT	AAATGGTAGACACGCGAGAC	45.0	50.0	57.8	58.0	564 bp	1.0	78.2