Hotspot

psbM-trnD-GUC

LSC · 10614-11258 · 645 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 645
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GCAGTAGCAATAAAGGCAAGA	AGATCCCTTCTGATTCCCCC	42.9	55.0	56.6	58.2	775 bp	1.0	59.4
psbM-trnD-GUC_p2	GCAGTAGCAATAAAGGCAAGA	GATCCCTTCTGATTCCCCCT	42.9	55.0	56.6	58.2	774 bp	1.0	59.4
psbM-trnD-GUC_p3	GCAGTAGCAATAAAGGCAAGA	TTCAATAGGCAAGAGCACCG	42.9	50.0	56.6	58.3	744 bp	1.0	58.7
psbM-trnD-GUC_p4	GCAGTAGCAATAAAGGCAAGAA	AGATCCCTTCTGATTCCCCC	40.9	55.0	57.3	58.2	775 bp	1.0	57.0
psbM-trnD-GUC_p5	GCAGTAGCAATAAAGGCAAGAA	GATCCCTTCTGATTCCCCCT	40.9	55.0	57.3	58.2	774 bp	1.0	57.0