Hotspot

trnK-UUU-rps16

LSC · 4362-5142 · 781 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
781
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 917 bp 1.0 76.1
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 916 bp 1.0 76.1
trnK-UUU-rps16_p3 TCAGTCGTGGTCTTCCAAAC TTCCTTGAAAAAGGCGCTCA 50.0 45.0 57.8 58.3 1002 bp 1.0 75.6
trnK-UUU-rps16_p4 CAGTCGTGGTCTTCCAAACT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 57.8 58.3 1001 bp 1.0 75.6
trnK-UUU-rps16_p5 TCCTAGCCGCACTTAAAAGC TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.3 58.3 932 bp 1.0 75.4