Hotspot

matK-trnK-UUU

LSC · 3578-4324 · 747 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
747
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 AGCCCTAAAGAGCTTTGGAA AACGGTAGAGTACTCGGCTT 45.0 50.0 56.7 58.2 800 bp 1.0 68.4
matK-trnK-UUU_p2 AGCCCTAAAGAGCTTTGGAA ACGGTAGAGTACTCGGCTTT 45.0 50.0 56.7 58.2 799 bp 1.0 68.4
matK-trnK-UUU_p3 AGCCCTAAAGAGCTTTGGAA TTGGGTTGCTAACTCAACGG 45.0 50.0 56.7 58.4 815 bp 1.0 66.6
matK-trnK-UUU_p4 AGCCCTAAAGAGCTTTGGAA GGGTTGCTAACTCAACGGTA 45.0 50.0 56.7 57.5 813 bp 1.0 66.1
matK-trnK-UUU_p5 AGCCCTAAAGAGCTTTGGAAT ACGGTAGAGTACTCGGCTTT 42.9 50.0 57.3 58.2 799 bp 1.0 64.7