primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	78	466	507-507	1.000000	76.3
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.469587	78	466	521-521	1.000000	76.2
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	78	466	520-520	1.000000	76.2
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	78	466	522-522	1.000000	76.2
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	20	20	55.0	50.0	58.1	57.6	0.0	0.0	0.525510	78	466	508-508	1.000000	75.8
psbA_p1	psbA	1	GAGCAATAACGCCCTCTTGA	TTGAAAATTCGTGCGCTTGG	20	20	50.0	45.0	58.0	58.2	0.0	9.7	0.259325	467	1528	1203-1203	1.000000	77.9
psbA_p2	psbA	2	AAAAGGAGCAATAACGCCCT	TTGAAAATTCGTGCGCTTGG	20	20	45.0	45.0	57.8	58.2	0.0	9.7	0.451091	467	1528	1208-1208	1.000000	76.4
psbA_p3	psbA	3	GAGCAATAACGCCCTCTTGA	ATATTGGTTGACACGGGCAT	20	20	50.0	45.0	58.0	57.6	0.0	0.0	0.459548	467	1528	1268-1268	1.000000	76.3
psbA_p4	psbA	4	GAGCAATAACGCCCTCTTGA	ATTGGTTGACACGGGCATAT	20	20	50.0	45.0	58.0	57.6	0.0	0.0	0.459548	467	1528	1266-1266	1.000000	76.3
psbA_p5	psbA	5	AAAGGAGCAATAACGCCCTC	TTGAAAATTCGTGCGCTTGG	20	20	50.0	45.0	58.2	58.2	0.0	9.7	0.483712	467	1528	1207-1207	1.000000	76.1
matK_p1	matK	1	ACATACTACATCAACATTTCAGA	ACCCCCAAAATCCTCTACCT	23	20	30.4	50.0	53.5	57.6	0.0	0.0	7.867308	2057	3577	1680-1681	1.000000	40.0
matK_p2	matK	2	ACATACTACATCAACATTTCAGAA	ACCCCCAAAATCCTCTACCT	24	20	29.2	50.0	54.3	57.6	0.0	0.0	8.080774	2057	3577	1680-1681	1.000000	40.0
matK_p3	matK	3	ACATACTACATCAACATTTCAGAAT	ACCCCCAAAATCCTCTACCT	25	20	28.0	50.0	54.8	57.6	0.0	0.0	8.549497	2057	3577	1680-1681	1.000000	40.0
matK_p4	matK	4	CTACATACTACATCAACATTTCAGA	ACCCCCAAAATCCTCTACCT	25	20	32.0	50.0	54.7	57.6	0.0	0.0	8.661903	2057	3577	1682-1683	1.000000	40.0
matK_p5	matK	5	ACATACTACATCAACATTTCAGA	AACCCCCAAAATCCTCTACC	23	20	30.4	50.0	53.5	56.8	0.0	0.0	8.707648	2057	3577	1681-1682	1.000000	40.0
matK-trnK-UUU_p1	matK-trnK-UUU	1	AGCCCTAAAGAGCTTTGGAA	AACGGTAGAGTACTCGGCTT	20	20	45.0	50.0	56.7	58.2	7.8	17.1	1.444067	3578	4324	800-800	1.000000	68.4
matK-trnK-UUU_p2	matK-trnK-UUU	2	AGCCCTAAAGAGCTTTGGAA	ACGGTAGAGTACTCGGCTTT	20	20	45.0	50.0	56.7	58.2	7.8	17.1	1.444067	3578	4324	799-799	1.000000	68.4
matK-trnK-UUU_p3	matK-trnK-UUU	3	AGCCCTAAAGAGCTTTGGAA	TTGGGTTGCTAACTCAACGG	20	20	45.0	50.0	56.7	58.4	7.8	9.4	1.675624	3578	4324	815-815	1.000000	66.6
matK-trnK-UUU_p4	matK-trnK-UUU	4	AGCCCTAAAGAGCTTTGGAA	GGGTTGCTAACTCAACGGTA	20	20	45.0	50.0	56.7	57.5	7.8	0.0	1.736404	3578	4324	813-813	1.000000	66.1
matK-trnK-UUU_p5	matK-trnK-UUU	5	AGCCCTAAAGAGCTTTGGAAT	ACGGTAGAGTACTCGGCTTT	21	20	42.9	50.0	57.3	58.2	7.8	17.1	1.915211	3578	4324	799-799	1.000000	64.7
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4362	5142	917-917	1.000000	76.1
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4362	5142	916-916	1.000000	76.1
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCAGTCGTGGTCTTCCAAAC	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	57.8	58.3	0.0	7.6	0.547945	4362	5142	1002-1002	1.000000	75.6
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CAGTCGTGGTCTTCCAAACT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	57.8	58.3	0.0	7.6	0.554139	4362	5142	1001-1001	1.000000	75.6
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCCTAGCCGCACTTAAAAGC	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.3	58.3	0.0	7.6	0.579483	4362	5142	932-932	1.000000	75.4
rps16_p1	rps16	1	ACGAATAGAGCAAGAAAAGAAGG	TCTCTGTACCGACTGAACCA	23	20	39.1	50.0	56.9	57.7	0.0	0.0	4.403459	5143	6250	1294-1294	1.000000	44.8
rps16_p2	rps16	2	ACGAATAGAGCAAGAAAAGAAGGA	TCTCTGTACCGACTGAACCA	24	20	37.5	50.0	58.2	57.7	0.0	0.0	4.486645	5143	6250	1294-1294	1.000000	44.1
rps16_p3	rps16	3	CGAATAGAGCAAGAAAAGAAGGA	TCTCTGTACCGACTGAACCA	23	20	39.1	50.0	56.6	57.7	0.0	0.0	4.654102	5143	6250	1293-1293	1.000000	42.8
rps16_p4	rps16	4	ACGAATAGAGCAAGAAAAGAAGG	GTCTCTGTACCGACTGAACC	23	20	39.1	55.0	56.9	57.4	0.0	0.0	4.755119	5143	6250	1295-1295	1.000000	42.0
rps16_p5	rps16	5	ACGAATAGAGCAAGAAAAGAAGGA	GTCTCTGTACCGACTGAACC	24	20	37.5	55.0	58.2	57.4	0.0	0.0	4.838305	5143	6250	1295-1295	1.000000	41.3
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6251	7190	1013-1013	1.000000	78.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	7.8	16.9	0.445953	6251	7190	1028-1028	1.000000	76.4
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6251	7190	1027-1027	1.000000	76.4
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CCAAGAGCACCTTCATTCCT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.5	58.1	0.0	16.9	0.579996	6251	7190	1084-1084	1.000000	75.4
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6251	7190	1017-1017	1.000000	73.5
trnS-GCU-trnC-GCA_p1	trnS-GCU-trnC-GCA	1	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.233476	8566	9338	924-924	1.000000	78.1
trnS-GCU-trnC-GCA_p2	trnS-GCU-trnC-GCA	2	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.2	58.4	0.0	14.6	0.506839	8566	9338	906-906	1.000000	75.9
trnS-GCU-trnC-GCA_p3	trnS-GCU-trnC-GCA	3	CTTTCGTCCACTCAGCCATC	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	58.6	58.1	0.0	0.0	0.718384	8566	9338	860-860	1.000000	74.3
trnS-GCU-trnC-GCA_p4	trnS-GCU-trnC-GCA	4	AACGGAAAGAGAGGGATTCG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.3	58.1	0.0	0.0	0.756923	8566	9338	925-925	1.000000	73.9
trnS-GCU-trnC-GCA_p5	trnS-GCU-trnC-GCA	5	CTTTCGTCCACTCAGCCATC	AAAAGGATTTGCAGTCCCCC	20	20	55.0	50.0	58.6	58.4	0.0	14.6	0.991747	8566	9338	842-842	1.000000	72.1
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	GCAGTAGCAATAAAGGCAAGA	AGATCCCTTCTGATTCCCCC	21	20	42.9	55.0	56.6	58.2	0.0	0.0	2.578056	10614	11258	775-775	1.000000	59.4
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	GCAGTAGCAATAAAGGCAAGA	GATCCCTTCTGATTCCCCCT	21	20	42.9	55.0	56.6	58.2	0.0	0.0	2.578056	10614	11258	774-774	1.000000	59.4
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	GCAGTAGCAATAAAGGCAAGA	TTCAATAGGCAAGAGCACCG	21	20	42.9	50.0	56.6	58.3	0.0	0.0	2.666665	10614	11258	744-744	1.000000	58.7
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	GCAGTAGCAATAAAGGCAAGAA	AGATCCCTTCTGATTCCCCC	22	20	40.9	55.0	57.3	58.2	0.0	0.0	2.875508	10614	11258	775-775	1.000000	57.0
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	GCAGTAGCAATAAAGGCAAGAA	GATCCCTTCTGATTCCCCCT	22	20	40.9	55.0	57.3	58.2	0.0	0.0	2.875508	10614	11258	774-774	1.000000	57.0
ycf4_p1	ycf4	1	TGCGAGTTACTTCGGAAACA	CGTTTTTACCCTCCTGCTGA	20	20	45.0	50.0	57.5	57.8	6.4	0.0	0.691397	59409	59963	687-687	1.000000	74.6
ycf4_p2	ycf4	2	TGCGAGTTACTTCGGAAACA	AGCATTGATTTCTCGGCTCA	20	20	45.0	45.0	57.5	57.6	6.4	0.0	0.920756	59409	59963	664-664	1.000000	72.8
ycf4_p3	ycf4	3	TGATGCGAGTTACTTCGGAA	CGTTTTTACCCTCCTGCTGA	20	20	45.0	50.0	57.0	57.8	3.3	0.0	1.200833	59409	59963	690-690	1.000000	70.5
ycf4_p4	ycf4	4	TTGATGCGAGTTACTTCGGA	CGTTTTTACCCTCCTGCTGA	20	20	45.0	50.0	57.0	57.8	6.4	0.0	1.200833	59409	59963	691-691	1.000000	70.5
ycf4_p5	ycf4	5	ATGCGAGTTACTTCGGAAACA	CGTTTTTACCCTCCTGCTGA	21	20	42.9	50.0	58.0	57.8	3.3	0.0	1.213633	59409	59963	688-688	1.000000	70.4
petA-psbJ_p1	petA-psbJ	1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	58.0	57.6	17.6	4.1	0.411974	62704	63518	952-953	1.000000	76.8
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTCGC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.9	57.6	0.0	4.1	0.416851	62704	63518	942-943	1.000000	76.8
petA-psbJ_p3	petA-psbJ	3	ACGTGTACAAGGCCTTTTGT	TGTGATCGGTTTAGTAGGCG	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.619192	62704	63518	990-991	1.000000	75.1
petA-psbJ_p4	petA-psbJ	4	ACGTGTACAAGGCCTTTTGT	GTGATCGGTTTAGTAGGCGT	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.620943	62704	63518	989-990	1.000000	75.1
petA-psbJ_p5	petA-psbJ	5	GGCCTTTTGTTCTTCTTCGC	TGTGATCGGTTTAGTAGGCG	20	20	50.0	50.0	57.9	57.4	0.0	0.0	0.624069	62704	63518	980-981	1.000000	75.1
ycf2_p1	ycf2	1	GGATGCTTAGCGGGTCATAG	ACATTCCGTACCTTGCGAAA	20	20	55.0	45.0	57.9	57.8	0.0	0.0	0.258905	86018	87017	1229-1229	1.000000	77.9
ycf2_p2	ycf2	2	TGCTTAGCGGGTCATAGTGA	ACATTCCGTACCTTGCGAAA	20	20	50.0	45.0	58.5	57.8	0.0	0.0	0.692062	86018	87017	1226-1226	1.000000	74.5
ycf2_p3	ycf2	3	ATGCTTAGCGGGTCATAGTG	ACATTCCGTACCTTGCGAAA	20	20	50.0	45.0	57.5	57.8	0.0	0.0	0.706117	86018	87017	1227-1227	1.000000	74.4
ycf2_p4	ycf2	4	TGGATGCTTAGCGGGTCATA	ACATTCCGTACCTTGCGAAA	20	20	50.0	45.0	58.6	57.8	0.0	0.0	0.753470	86018	87017	1230-1230	1.000000	74.0
ycf2_p5	ycf2	5	GCTTAGCGGGTCATAGTGAA	ACATTCCGTACCTTGCGAAA	20	20	50.0	45.0	57.4	57.8	0.0	0.0	0.769755	86018	87017	1225-1225	1.000000	73.8
ycf2-trnL-CAA_p1	ycf2-trnL-CAA	1	GCTTTTCCCGGATGAAATGC	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	58.1	58.0	20.3	15.9	0.081269	92891	93298	562-562	1.000000	79.5
ycf2-trnL-CAA_p2	ycf2-trnL-CAA	2	GCTTTTCCCGGATGAAATGC	TGAAATGGTAGACACGCGAG	20	20	50.0	50.0	58.1	58.0	20.3	15.9	0.082640	92891	93298	564-564	1.000000	79.5
ycf2-trnL-CAA_p3	ycf2-trnL-CAA	3	GCTTTTCCCGGATGAAATGC	GCCTTGGTGGTGAAATGGTA	20	20	50.0	50.0	58.1	58.1	20.3	0.0	0.155567	92891	93298	574-574	1.000000	78.9
ycf2-trnL-CAA_p4	ycf2-trnL-CAA	4	ATGGCTTTTCCCGGATGAAA	AAATGGTAGACACGCGAGAC	20	20	45.0	50.0	57.8	58.0	0.7	15.9	0.237960	92891	93298	565-565	1.000000	78.2
ycf2-trnL-CAA_p5	ycf2-trnL-CAA	5	TGGCTTTTCCCGGATGAAAT	AAATGGTAGACACGCGAGAC	20	20	45.0	50.0	57.8	58.0	0.7	15.9	0.237960	92891	93298	564-564	1.000000	78.2
ycf1_p1	ycf1	1	TCCCCTTTTGAATGGGATGT	TATGACCATCGAGGGGGTTT	20	20	45.0	50.0	56.7	58.1	14.5	21.9	1.431878	109830	109949	306-306	1.000000	68.8
ycf1_p2	ycf1	2	TGAATGGGATGTATCGTGGA	TATGACCATCGAGGGGGTTT	20	20	45.0	50.0	56.0	58.1	0.0	21.9	2.094390	109830	109949	298-298	1.000000	63.5
ycf1_p3	ycf1	3	TGGGATGTATCGTGGAAGAA	TATGACCATCGAGGGGGTTT	20	20	45.0	50.0	55.9	58.1	0.0	21.9	2.166464	109830	109949	294-294	1.000000	62.9
ycf1_p4	ycf1	4	TGAATGGGATGTATCGTGGAAG	TATGACCATCGAGGGGGTTT	22	20	45.5	50.0	57.9	58.1	0.0	21.9	2.184163	109830	109949	298-298	1.000000	62.8
ycf1_p5	ycf1	5	TGGGATGTATCGTGGAAGAATG	TATGACCATCGAGGGGGTTT	22	20	45.5	50.0	57.9	58.1	0.0	21.9	2.184163	109830	109949	294-294	1.000000	62.8
ccsA_p1	ccsA	1	AGAACCGCGTGAATCACTAC	TCTTAATTAATCTAGGGGATTCC	20	23	50.0	34.8	58.0	52.8	11.9	0.0	8.240946	122010	122978	1126-1126	1.000000	40.1
ccsA_p2	ccsA	2	AGAACCGCGTGAATCACTAC	TTCTTAATTAATCTAGGGGATTCC	20	24	50.0	33.3	58.0	53.6	11.9	0.0	8.411677	122010	122978	1127-1127	1.000000	40.1
ccsA_p3	ccsA	3	TGATTCACATGGTTCTCGCC	TCTTAATTAATCTAGGGGATTCC	20	23	50.0	34.8	58.3	52.8	0.0	0.0	8.495277	122010	122978	1100-1100	1.000000	40.1
ccsA_p4	ccsA	4	GAGAACCGCGTGAATCACTA	TCTTAATTAATCTAGGGGATTCC	20	23	50.0	34.8	57.7	52.8	11.9	0.0	8.506485	122010	122978	1127-1127	1.000000	40.1
ccsA_p5	ccsA	5	GAACCGCGTGAATCACTACA	TCTTAATTAATCTAGGGGATTCC	20	23	50.0	34.8	58.3	52.8	11.9	0.0	8.527859	122010	122978	1125-1125	1.000000	40.1
trnL-CAA-ycf2_p1	trnL-CAA-ycf2	1	AAATGGTAGACACGCGAGAC	GCTTTTCCCGGATGAAATGC	20	20	50.0	50.0	58.0	58.1	15.9	20.3	0.081269	143173	143580	562-562	1.000000	79.5
trnL-CAA-ycf2_p2	trnL-CAA-ycf2	2	TGAAATGGTAGACACGCGAG	GCTTTTCCCGGATGAAATGC	20	20	50.0	50.0	58.0	58.1	15.9	20.3	0.082640	143173	143580	564-564	1.000000	79.5
trnL-CAA-ycf2_p3	trnL-CAA-ycf2	3	GCCTTGGTGGTGAAATGGTA	GCTTTTCCCGGATGAAATGC	20	20	50.0	50.0	58.1	58.1	0.0	20.3	0.155567	143173	143580	574-574	1.000000	78.9
trnL-CAA-ycf2_p4	trnL-CAA-ycf2	4	AAATGGTAGACACGCGAGAC	ATGGCTTTTCCCGGATGAAA	20	20	50.0	45.0	58.0	57.8	15.9	0.7	0.237960	143173	143580	565-565	1.000000	78.2
trnL-CAA-ycf2_p5	trnL-CAA-ycf2	5	AAATGGTAGACACGCGAGAC	TGGCTTTTCCCGGATGAAAT	20	20	50.0	45.0	58.0	57.8	15.9	0.7	0.237960	143173	143580	564-564	1.000000	78.2