Hotspot

rpl16

LSC · 84505-85933 · 1429 bp

Back to Archiphysalis

Pi 0.0014

MarkerSeek score 59.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1429
variable_sites: 2
pi: 0.0014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AACATGCGGTTTTGTTTCAT	TCGATTGACCATTTGATATAATTGCT	35.0	30.8	55.0	57.6	1510 bp	1.0	40.1
rpl16_p2	AACATGCGGTTTTGTTTCAT	TCGATTGACCATTTGATATAATTGC	35.0	32.0	55.0	56.4	1510 bp	1.0	40.1
rpl16_p3	AACATGCGGTTTTGTTTCAT	CGATTGACCATTTGATATAATTGCT	35.0	32.0	55.0	56.4	1509 bp	1.0	40.1
rpl16_p4	AACATGCGGTTTTGTTTCAT	AAAATTCGATTGACCATTTGA	35.0	28.6	55.0	52.3	1515 bp	1.0	40.1
rpl16_p5	AACATGCGGTTTTGTTTCAT	AAAAATTCGATTGACCATTTGA	35.0	27.3	55.0	53.2	1516 bp	1.0	40.1