Hotspot

rbcL

LSC · 57805-59238 · 1434 bp

Back to Archiphysalis

Pi 0.000697

MarkerSeek score 59.20

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1434
variable_sites: 1
pi: 0.000697
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TAATGCGGAATTCGTGTCGA	TGGGCCGAGTTTAATTGCAA	45.0	45.0	57.7	58.4	1618 bp	1.0	74.8
rbcL_p2	TAATGCGGAATTCGTGTCGA	TTGGGCCGAGTTTAATTGCA	45.0	45.0	57.7	58.4	1619 bp	1.0	74.8
rbcL_p3	GCGGAATTCGTGTCGAGTAG	TGGGCCGAGTTTAATTGCAA	55.0	45.0	58.8	58.4	1614 bp	1.0	70.5
rbcL_p4	GCGGAATTCGTGTCGAGTAG	TTGGGCCGAGTTTAATTGCA	55.0	45.0	58.8	58.4	1615 bp	1.0	70.5
rbcL_p5	CGGAATTCGTGTCGAGTAGA	TGGGCCGAGTTTAATTGCAA	50.0	45.0	57.2	58.4	1613 bp	1.0	70.5