Hotspot

ccsA

SSC · 116982-117932 · 951 bp

Back to Archiphysalis

Pi 0.00105

MarkerSeek score 59.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 951
variable_sites: 1
pi: 0.00105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGCATACCGTCTTCTAAAAAGG	CCGTCCGCTCTTGTATTTGT	45.5	50.0	57.1	58.3	1094-1097 bp	1.0	54.8
ccsA_p2	GGCATACCGTCTTCTAAAAAGG	TTGATGATTCACACGGTCCG	45.5	50.0	57.1	58.3	1140-1143 bp	1.0	54.8
ccsA_p3	GGCATACCGTCTTCTAAAAAGGA	CCGTCCGCTCTTGTATTTGT	43.5	50.0	58.5	58.3	1094-1097 bp	1.0	49.9
ccsA_p4	GGCATACCGTCTTCTAAAAAGGA	TTGATGATTCACACGGTCCG	43.5	50.0	58.5	58.3	1140-1143 bp	1.0	49.9
ccsA_p5	TGAATTCAATTCCCGATTTCCA	CCGTCCGCTCTTGTATTTGT	36.4	50.0	56.3	58.3	1055-1058 bp	1.0	48.3