Hotspot

petA-psbJ

LSC · 66041-67119 · 1079 bp

Back to Archiphysalis

Pi 0.000934

MarkerSeek score 60.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1079
variable_sites: 1
pi: 0.000934
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.2	58.3	1138-1145 bp	1.0	76.7
petA-psbJ_p2	TCGAGAAGGTTCAATTGGCC	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.2	58.3	1140-1147 bp	1.0	76.7
petA-psbJ_p3	AGAAGGTTCAATTGGCCGAA	GGTTCATATTCCGGGTTGGG	45.0	55.0	57.7	58.3	1137-1144 bp	1.0	75.8
petA-psbJ_p4	GAGAAGGTTCAATTGGCCGA	CATATTCCGGGTTGGGTTCA	50.0	50.0	58.2	57.6	1134-1141 bp	1.0	75.8
petA-psbJ_p5	GAGAAGGTTCAATTGGCCGA	TGGTTCATATTCCGGGTTGG	50.0	50.0	58.2	57.6	1139-1146 bp	1.0	75.8