Hotspot

psbM-trnD-GUC

LSC · 30819-31898 · 1080 bp

Back to Archiphysalis

Pi 0.00278

MarkerSeek score 60.30

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1080
variable_sites: 3
pi: 0.00278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGTAGAAAAGCGGTAGGAACT	CCCGTATTTCCCTGGGATTG	42.9	55.0	56.4	58.0	1235-1236 bp	1.0	59.2
psbM-trnD-GUC_p2	AGTAGAAAAGCGGTAGGAACT	TCCCGTATTTCCCTGGGATT	42.9	50.0	56.4	58.1	1236-1237 bp	1.0	58.5
psbM-trnD-GUC_p3	AAGTAGAAAAGCGGTAGGAACT	CCCGTATTTCCCTGGGATTG	40.9	55.0	57.1	58.0	1236-1237 bp	1.0	57.0
psbM-trnD-GUC_p4	AGAAAAGCGGTAGGAACTAGA	CCCGTATTTCCCTGGGATTG	42.9	55.0	56.1	58.0	1232-1233 bp	1.0	56.9
psbM-trnD-GUC_p5	AAGTAGAAAAGCGGTAGGAACT	TCCCGTATTTCCCTGGGATT	40.9	50.0	57.1	58.1	1237-1238 bp	1.0	56.3