Hotspot

atpH-atpI

LSC · 13930-15149 · 1220 bp

Back to Archiphysalis

Pi 0.00082

MarkerSeek score 59.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1220
variable_sites: 1
pi: 0.00082
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TACCTGTCATGCTCCTTGGA	45.0	50.0	58.0	58.0	1356-1363 bp	1.0	79.4
atpH-atpI_p2	TACCCTCTACAGCTTGACCC	TACCTGTCATGCTCCTTGGA	55.0	50.0	58.1	58.0	1439-1446 bp	1.0	78.6
atpH-atpI_p3	GCGATACCCTCTACAGCTTG	TACCTGTCATGCTCCTTGGA	55.0	50.0	57.9	58.0	1443-1450 bp	1.0	78.5
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TACCTGTCATGCTCCTTGGA	45.0	50.0	58.2	58.0	1357-1364 bp	1.0	78.2
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	TACCTGTCATGCTCCTTGGA	50.0	50.0	57.8	58.0	1371-1378 bp	1.0	78.0