Hotspot

trnS-GCU-trnG-GCC

LSC · 8707-9320 · 614 bp

Back to Archiphysalis

Pi 0.00163

MarkerSeek score 59.70

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 614
variable_sites: 1
pi: 0.00163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	688 bp	1.0	51.5
trnS-GCU-trnG-GCC_p2	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	737 bp	1.0	50.6
trnS-GCU-trnG-GCC_p3	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	45.0	39.1	58.0	57.3	691 bp	1.0	50.5
trnS-GCU-trnG-GCC_p4	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	45.0	39.1	58.0	57.3	690 bp	1.0	50.5
trnS-GCU-trnG-GCC_p5	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	45.0	36.4	58.0	56.2	689 bp	1.0	49.6