Hotspot

ccsA

SSC · 135968-136939 · 972 bp

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Pi 0.00617

MarkerSeek score 47.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 6
pi: 0.00617
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	GGTGTTTGTGAGAACCCCTT	50.0	50.0	58.0	57.9	1514-1576 bp	1.0	84.1
ccsA_p2	TGAAATCGGTAGACACGCTG	GGTGTTTGTGAGAACCCCTT	50.0	50.0	58.0	57.9	1513-1575 bp	1.0	84.1
ccsA_p3	GTGAAATCGGTAGACACGCT	TGGGCCCCGAGAGTTATTTA	50.0	50.0	58.0	58.1	1795-1858 bp	1.0	83.1
ccsA_p4	TGAAATCGGTAGACACGCTG	TGGGCCCCGAGAGTTATTTA	50.0	50.0	58.0	58.1	1794-1857 bp	1.0	83.1
ccsA_p5	GTGAAATCGGTAGACACGCT	CAGTTGACAAGGTCGAAGCT	50.0	50.0	58.0	58.1	1709-1772 bp	1.0	83.1