Hotspot

ccsA-ndhD

IRa · 136940-137478 · 539 bp

Back to Archidendron

Pi 0.127

MarkerSeek score 85.10

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: IRa
length_bp: 539
variable_sites: 66
pi: 0.127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TCAATAGTGGCCTCTATGGG	CAGTTGACAAGGTCGAAGCT	50.0	50.0	56.1	58.1	685-747 bp	1.0	73.2
ccsA-ndhD_p2	TCAATAGTGGCCTCTATGGG	TCAGTTGACAAGGTCGAAGC	50.0	50.0	56.1	58.1	686-748 bp	1.0	73.1
ccsA-ndhD_p3	CTTCAATAGTGGCCTCTATGGG	CAGTTGACAAGGTCGAAGCT	50.0	50.0	58.0	58.1	687-749 bp	1.0	72.4
ccsA-ndhD_p4	CTTCAATAGTGGCCTCTATGGG	TCAGTTGACAAGGTCGAAGC	50.0	50.0	58.0	58.1	688-750 bp	1.0	72.4
ccsA-ndhD_p5	TTCAATAGTGGCCTCTATGGG	CAGTTGACAAGGTCGAAGCT	47.6	50.0	56.9	58.1	686-748 bp	1.0	71.4