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rps3-rps19

LSC · 91854-92325 · 472 bp

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Pi 0.0953

MarkerSeek score 81.10

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 472
variable_sites: 43
pi: 0.0953
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	TTATCCGTGCAATTCCCTCG	ATAAACTTCCGGGGACATGC	50.0	50.0	58.1	57.9	710-896 bp	1.0	85.6
rps3-rps19_p2	GGTGTCCATAAGGGTTTGCA	ATAAACTTCCGGGGACATGC	50.0	50.0	58.1	57.9	890-1076 bp	1.0	84.2
rps3-rps19_p3	ACCTTCTCTGATCCAACGGA	ATAAACTTCCGGGGACATGC	50.0	50.0	58.1	57.9	1066-1252 bp	1.0	83.8
rps3-rps19_p4	TTATCCGTGCAATTCCCTCG	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.1	58.1	1128-1313 bp	1.0	83.7
rps3-rps19_p5	ACCTTCTCTGATCCAACGGA	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.1	58.1	1484-1669 bp	1.0	82.7