Hotspot

rpl33-rps18

LSC · 75393-75635 · 243 bp

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Pi 0.0494

MarkerSeek score 68.60

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 243
variable_sites: 12
pi: 0.0494
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	TCTGTCCCTGTTGTTGCAAA	CTTGGATTTAAGAAAAAGTCGCT	45.0	34.8	57.9	55.8	329-413 bp	1.0	42.6
rpl33-rps18_p2	TTCTGTCCCTGTTGTTGCAA	CTTGGATTTAAGAAAAAGTCGCT	45.0	34.8	57.9	55.8	330-414 bp	1.0	42.6
rpl33-rps18_p3	TCTGTCCCTGTTGTTGCAAA	TGGATTTAAGAAAAAGTCGCT	45.0	33.3	57.9	53.7	327-411 bp	1.0	42.6
rpl33-rps18_p4	TTCTGTCCCTGTTGTTGCAA	TGGATTTAAGAAAAAGTCGCT	45.0	33.3	57.9	53.7	328-412 bp	1.0	42.6
rpl33-rps18_p5	TCTGTCCCTGTTGTTGCAAA	TGGATTTAAGAAAAAGTCGCTT	45.0	31.8	57.9	54.6	327-411 bp	1.0	42.6