Hotspot

rbcL-accD

LSC · 62939-64061 · 1123 bp

Back to Archidendron

Pi 0.0095

MarkerSeek score 65.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 1123
variable_sites: 9
pi: 0.0095
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AGTCCTGAATTAGCTGCTGC	TGGGTCTTCACTTACACTGG	50.0	50.0	58.0	56.8	1145-1318 bp	1.0	74.5
rbcL-accD_p2	AGTCCTGAATTAGCTGCTGC	GGGTCTTCACTTACACTGGT	50.0	50.0	58.0	56.8	1144-1317 bp	1.0	74.5
rbcL-accD_p3	GAGGCTAGCAAATGGAGTCC	TGGGTCTTCACTTACACTGG	55.0	50.0	58.0	56.8	1160-1333 bp	1.0	74.5
rbcL-accD_p4	GAGGCTAGCAAATGGAGTCC	GGGTCTTCACTTACACTGGT	55.0	50.0	58.0	56.8	1159-1332 bp	1.0	74.5
rbcL-accD_p5	TTCGTGAGGCTAGCAAATGG	TGGGTCTTCACTTACACTGG	50.0	50.0	58.3	56.8	1165-1338 bp	1.0	72.7