Hotspot

trnT-UGU-trnL-UAA

LSC · 51961-53498 · 1538 bp

Back to Archidendron

Pi 0.022

MarkerSeek score 73.40

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1538
variable_sites: 33
pi: 0.022
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	50.0	50.0	57.8	58.0	1599-1620 bp	1.0	81.4
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	1607-1628 bp	1.0	80.9
trnT-UGU-trnL-UAA_p3	AAATGCGACGCTCTAACCTC	ACCAATTTCGCCATATCCCC	50.0	50.0	58.4	58.0	1571-1592 bp	1.0	80.5
trnT-UGU-trnL-UAA_p4	AAATGCGACGCTCTAACCTC	TAGCGTCTACCAATTTCGCC	50.0	50.0	58.4	58.1	1579-1600 bp	1.0	80.0
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.8	58.2	1613-1634 bp	1.0	79.5