Hotspot

psbZ-trnG-GCC

LSC · 41433-42034 · 602 bp

Back to Archidendron

Pi 0.0247

MarkerSeek score 71.80

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 602
variable_sites: 14
pi: 0.0247
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	GCGTCTTCTCTTTGGCAAAG	55.0	50.0	58.0	57.7	757-837 bp	1.0	82.2
psbZ-trnG-GCC_p2	CTTCTCCTGATGGTTGGTCG	ATACATAAATAGGGGGCGCG	55.0	50.0	58.0	57.6	801-881 bp	1.0	81.4
psbZ-trnG-GCC_p3	TTGCTTCTCCTGATGGTTGG	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	57.8	57.7	760-840 bp	1.0	80.7
psbZ-trnG-GCC_p4	TCCTGATGGTTGGTCGAGTA	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	57.8	57.7	753-833 bp	1.0	80.6
psbZ-trnG-GCC_p5	GCTTCTCCTGATGGTTGGTC	GCGTCTTCTCTTTGGCAAAG	55.0	50.0	58.3	57.7	758-838 bp	1.0	80.3