Hotspot

trnS-UGA-psbZ

LSC · 40913-41243 · 331 bp

Back to Archidendron

Pi 0.00606

MarkerSeek score 46.60

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 331
variable_sites: 2
pi: 0.00606
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	TGCAAAAACAGCCAATTGGA	55.0	40.0	58.0	57.0	385-388 bp	1.0	72.5
trnS-UGA-psbZ_p2	GAGCTATCAACCACTCAGCC	TGCAAAAACAGCCAATTGGA	55.0	40.0	58.1	57.0	392-395 bp	1.0	72.2
trnS-UGA-psbZ_p3	GGAGCTATCAACCACTCAGC	TGCAAAAACAGCCAATTGGA	55.0	40.0	58.1	57.0	393-396 bp	1.0	72.2
trnS-UGA-psbZ_p4	CAAGACCGGAGCTATCAACC	TGCAAAAACAGCCAATTGGA	55.0	40.0	58.1	57.0	400-403 bp	1.0	72.2
trnS-UGA-psbZ_p5	CGGAGCTATCAACCACTCAG	TGCAAAAACAGCCAATTGGA	55.0	40.0	57.8	57.0	394-397 bp	1.0	70.9