Hotspot

trnR-UCU-atpA

LSC · 11904-12447 · 544 bp

Back to Archidendron

Pi 0.0133

MarkerSeek score 64.30

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 544
variable_sites: 7
pi: 0.0133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CATTCACTCGGGAAGCAGAA	47.6	50.0	57.3	57.9	658 bp	1.0	68.7
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	AAGACATTCACTCGGGAAGC	47.6	50.0	57.3	57.9	662 bp	1.0	68.7
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	GGAAGCAGAAGCCCTTTTGA	47.6	50.0	57.3	58.4	648 bp	1.0	66.5
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	ATTCACTCGGGAAGCAGAAG	47.6	50.0	57.3	57.6	657 bp	1.0	66.4
trnR-UCU-atpA_p5	TGGATAGGACAGAGGTCTTCT	CATTCACTCGGGAAGCAGAA	47.6	50.0	56.9	57.9	693 bp	1.0	65.7