Hotspot

trnQ-UUG-psbK

LSC · 7548-8252 · 705 bp

Back to Archidendron

Pi 0.0345

MarkerSeek score 79.90

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 705
variable_sites: 24
pi: 0.0345
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	GATATGCTCTGGGACGGAAG	TAGGCCTCAGGTAATTTCGC	55.0	50.0	57.6	57.4	874-881 bp	1.0	74.6
trnQ-UUG-psbK_p2	GATATGCTCTGGGACGGAAG	GCCTCAGGTAATTTCGCGAA	55.0	50.0	57.6	58.6	871-878 bp	1.0	74.5
trnQ-UUG-psbK_p3	GACCAAAACCCGTTGCCTTA	TAGGCCTCAGGTAATTTCGC	50.0	50.0	58.7	57.4	832-839 bp	1.0	72.9
trnQ-UUG-psbK_p4	GACCAAAACCCGTTGCCTTA	GCCTCAGGTAATTTCGCGAA	50.0	50.0	58.7	58.6	829-836 bp	1.0	72.8
trnQ-UUG-psbK_p5	GATATGCTCTGGGACGGAAG	AAAAAGCATAGGCCTCAGGT	55.0	45.0	57.6	57.1	882-889 bp	1.0	72.2