Hotspot

trnK-UUU-rps16

LSC · 4366-5598 · 1233 bp

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Pi 0.0179
MarkerSeek score 60.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1233
variable_sites
21
pi
0.0179
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 GTATTTGGGCCCGATCCAAT TGAGGTTGCGAATACGAGTG 50.0 50.0 58.0 58.0 1863-1892 bp 1.0 83.6
trnK-UUU-rps16_p2 CCTTTCTGGATCAGTCGTGG TGAGGTTGCGAATACGAGTG 55.0 50.0 58.0 58.0 1784-1813 bp 1.0 83.6
trnK-UUU-rps16_p3 GTATTTGGGCCCGATCCAAT AAGCTAACGGATCGTGGAAC 50.0 50.0 58.0 58.0 2074-2103 bp 1.0 83.3
trnK-UUU-rps16_p4 CCTTTCTGGATCAGTCGTGG AAGCTAACGGATCGTGGAAC 55.0 50.0 58.0 58.0 1995-2024 bp 1.0 83.3
trnK-UUU-rps16_p5 ACATGTATTTGGGCCCGATC AAGCTAACGGATCGTGGAAC 50.0 50.0 58.0 58.0 2078-2107 bp 1.0 83.2