Hotspot

matK-trnK-UUU

LSC · 3593-4328 · 736 bp

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Pi 0.00951

MarkerSeek score 64.20

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 736
variable_sites: 7
pi: 0.00951
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	CCATGATCGTAAGCAAGTGC	TCAATGGTAGAGTACTCGGCT	50.0	47.6	57.3	58.0	869-870 bp	1.0	66.7
matK-trnK-UUU_p2	CCATGATCGTAAGCAAGTGC	AGGGTTGCTAACTCAATGGT	50.0	45.0	57.3	56.7	881-882 bp	1.0	64.6
matK-trnK-UUU_p3	CCATGATCGTAAGCAAGTGC	CTCAATGGTAGAGTACTCGGC	50.0	52.4	57.3	57.6	870-871 bp	1.0	63.9
matK-trnK-UUU_p4	CCATGATCGTAAGCAAGTGC	TCAATGGTAGAGTACTCGGC	50.0	50.0	57.3	56.5	869-870 bp	1.0	62.5
matK-trnK-UUU_p5	CCATGATCGTAAGCAAGTGC	CAATGGTAGAGTACTCGGCT	50.0	50.0	57.3	56.5	868-869 bp	1.0	62.4