Hotspot

rrn23

IRb · 103424-106233 · 2810 bp

Back to Archidasyphyllum

Pi 0.000712

MarkerSeek score 56.40

Primer available yes

Label rrn23

Feature Metadata

label_name: rrn23
feature_type: rRNA
region: IRb
length_bp: 2810
variable_sites: 2
pi: 0.000712
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn23_p1	TCCATCCATTTAGCAGCACC	AAACTTCTGTCGCTCCATCC	50.0	50.0	57.9	57.9	2949 bp	1.0	78.8
rrn23_p2	TCCATCCATTTAGCAGCACC	GCCGTGACCTTCTCTTGAAT	50.0	50.0	57.9	57.9	2974 bp	1.0	78.8
rrn23_p3	CCATCCATTTAGCAGCACCT	AAACTTCTGTCGCTCCATCC	50.0	50.0	57.9	57.9	2948 bp	1.0	78.7
rrn23_p4	CCATCCATTTAGCAGCACCT	GCCGTGACCTTCTCTTGAAT	50.0	50.0	57.9	57.9	2973 bp	1.0	78.7
rrn23_p5	CGGCGGTTCGATCTATGATT	AAACTTCTGTCGCTCCATCC	50.0	50.0	57.9	57.9	2993 bp	1.0	78.7