Hotspot

rps11

LSC · 78259-78669 · 411 bp

Back to Archidasyphyllum

Pi 0

MarkerSeek score 59.40

Primer available yes

Label rps11

Feature Metadata

label_name: rps11
feature_type: gene
region: LSC
length_bp: 411
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11_p1	ACTGTTACTTTCTCTCGAACCA	GGGGGATCTGTTTTGACATGA	40.9	47.6	57.1	57.9	560-584 bp	1.0	51.2
rps11_p2	ACTGTTACTTTCTCTCGAACCA	AGGGGGATCTGTTTTGACATG	40.9	47.6	57.1	57.9	561-585 bp	1.0	51.2
rps11_p3	ACTGTTACTTTCTCTCGAACCA	GAAGGGGGATCTGTTTTGACA	40.9	47.6	57.1	57.8	563-587 bp	1.0	50.6
rps11_p4	ACTGTTACTTTCTCTCGAACCA	AAGGGGGATCTGTTTTGACA	40.9	45.0	57.1	56.6	562-586 bp	1.0	48.9
rps11_p5	AGTGTCCGAGTAGATACTGT	GGGGGATCTGTTTTGACATGA	45.0	47.6	54.7	57.9	575-599 bp	1.0	48.4