Hotspot

clpP

LSC · 69231-71266 · 2036 bp

Back to Archidasyphyllum

Pi 0.000498

MarkerSeek score 59.20

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2036
variable_sites: 1
pi: 0.000498
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CGAATTGACGCGAAATCCAG	GCCTTTGTTACGTTTCCACA	50.0	45.0	57.9	56.9	2098-2125 bp	1.0	71.4
clpP_p2	CTCGGCAGGGGTAATTTCAA	GCCTTTGTTACGTTTCCACA	50.0	45.0	57.9	56.9	2122-2149 bp	1.0	71.0
clpP_p3	AACTCGGCAGGGGTAATTTC	GCCTTTGTTACGTTTCCACA	50.0	45.0	57.9	56.9	2124-2151 bp	1.0	71.0
clpP_p4	CACGAATTGACGCGAAATCC	GCCTTTGTTACGTTTCCACA	50.0	45.0	58.2	56.9	2100-2127 bp	1.0	70.4
clpP_p5	CGAATTGACGCGAAATCCAG	AGCCTTTGTTACGTTTCCACA	50.0	42.9	57.9	58.4	2099-2126 bp	1.0	69.6