Hotspot

ndhD-psaC

SSC · 115762-115880 · 119 bp

Back to Archidasyphyllum

Pi 0.0252

MarkerSeek score 67.00

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 119
variable_sites: 3
pi: 0.0252
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AGGAACCCGCAAATATTGGT	ACAGAGGATTGTGTCGGTTG	45.0	50.0	57.5	57.8	291 bp	1.0	75.3
ndhD-psaC_p2	AGGAACCCGCAAATATTGGT	CAGAGGATTGTGTCGGTTGT	45.0	50.0	57.5	57.8	290 bp	1.0	75.3
ndhD-psaC_p3	AGGAACCCGCAAATATTGGT	AAGAGATGTGAATCCGCCTG	45.0	50.0	57.5	57.7	270 bp	1.0	74.1
ndhD-psaC_p4	AGGAACCCGCAAATATTGGT	GAACAGAGGATTGTGTCGGT	45.0	50.0	57.5	57.5	293 bp	1.0	73.0
ndhD-psaC_p5	AGGAACCCGCAAATATTGGT	GCCCAACGGATTTCTTGAGT	45.0	50.0	57.5	58.5	251 bp	1.0	73.0