Hotspot

psbT-psbN

LSC · 73531-73603 · 73 bp

Back to Archidasyphyllum

Pi 0.0274

MarkerSeek score 64.80

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 73
variable_sites: 2
pi: 0.0274
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TAGAGAACCGCCGAAAGTTC	AGAGATCCGTTCGAGGAACA	50.0	50.0	57.6	58.2	137 bp	1.0	77.0
psbT-psbN_p2	TAGAGAACCGCCGAAAGTTC	TCGAGGAACATGGGGACTAA	50.0	50.0	57.6	57.8	127 bp	1.0	76.5
psbT-psbN_p3	TAGAGAACCGCCGAAAGTTC	TTCGAGGAACATGGGGACTA	50.0	50.0	57.6	57.8	128 bp	1.0	76.4
psbT-psbN_p4	CCGCCGAAAGTTCCAACTAA	AGAGATCCGTTCGAGGAACA	50.0	50.0	58.5	58.2	130 bp	1.0	76.0
psbT-psbN_p5	TAGAGAACCGCCGAAAGTTC	GGGTACGCCTTATATACCGC	50.0	55.0	57.6	57.7	182 bp	1.0	75.7