Hotspot

petA-psbJ

LSC · 62993-63760 · 768 bp

Back to Archidasyphyllum

Pi 0.0013

MarkerSeek score 59.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 768
variable_sites: 1
pi: 0.0013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTACAAGGCCTTTTGT	GGTTCATATTCCGGGTTGGG	45.0	55.0	58.0	58.3	913-914 bp	1.0	77.2
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	GGTTCATATTCCGGGTTGGG	50.0	55.0	57.9	58.3	903-904 bp	1.0	77.2
petA-psbJ_p3	ACGTGTACAAGGCCTTTTGT	CATATTCCGGGTTGGGTTCA	45.0	50.0	58.0	57.6	909-910 bp	1.0	76.3
petA-psbJ_p4	ACGTGTACAAGGCCTTTTGT	TGGTTCATATTCCGGGTTGG	45.0	50.0	58.0	57.6	914-915 bp	1.0	76.3
petA-psbJ_p5	ACGTGTACAAGGCCTTTTGT	GTTCATATTCCGGGTTGGGT	45.0	50.0	58.0	57.6	912-913 bp	1.0	76.3