Hotspot

atpH-atpI

LSC · 13897-14948 · 1052 bp

Back to Archidasyphyllum

Pi 0.000956

MarkerSeek score 59.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1052
variable_sites: 1
pi: 0.000956
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.0	58.3	1183-1188 bp	1.0	78.1
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TCCCTGTCATGTTCCTTGGA	50.0	50.0	57.9	58.3	1239-1244 bp	1.0	77.5
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	57.9	58.3	1199-1204 bp	1.0	77.2
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.2	58.3	1184-1189 bp	1.0	76.9
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	TCCCTGTCATGTTCCTTGGA	55.0	50.0	58.2	58.3	1243-1248 bp	1.0	76.9