Hotspot

trnK-UUU-rps16

LSC · 4365-5190 · 826 bp

Back to Archidasyphyllum

Pi 0.00242
MarkerSeek score 60.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
826
variable_sites
2
pi
0.00242
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCCCTCGCTTCATCCAAATG TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.0 58.3 1006 bp 1.0 77.3
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 1023 bp 1.0 77.0
trnK-UUU-rps16_p3 CGATGGTATGGACGAATCCC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 1022 bp 1.0 77.0
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 962 bp 1.0 76.3
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 961 bp 1.0 76.3