Hotspot

rpoC2

Genome · 46902-50507 · 3606 bp

Back to Araucaria

Pi 0.00499

MarkerSeek score 55.10

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: Genome
length_bp: 3606
variable_sites: 18
pi: 0.00499
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	GTGGTACGAGCGATCCATTT	TTGGAGGGGTGATACCTGTT	50.0	50.0	58.1	57.9	283-284 bp	1.0	79.9
rpoC2_p2	GGTACGAGCGATCCATTTCA	TTGGAGGGGTGATACCTGTT	50.0	50.0	57.8	57.9	281-282 bp	1.0	78.7
rpoC2_p3	TGGTACGAGCGATCCATTTC	TTGGAGGGGTGATACCTGTT	50.0	50.0	57.8	57.9	282-283 bp	1.0	78.7
rpoC2_p4	AAAGATTGCCTGCCCGTATC	TTGGAGGGGTGATACCTGTT	50.0	50.0	58.3	57.9	307-308 bp	1.0	77.8
rpoC2_p5	CAAAGATTGCCTGCCCGTAT	TTGGAGGGGTGATACCTGTT	50.0	50.0	58.6	57.9	308-309 bp	1.0	75.5