Hotspot

rpoA-rps11

Genome · 112733-112795 · 63 bp

Back to Araucaria

Pi 0.0317

MarkerSeek score 70.80

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: Genome
length_bp: 63
variable_sites: 2
pi: 0.0317
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	GCACTTCCATTGTGGTCTCT	AATGGCTGTAGACCTCCCAA	50.0	50.0	57.8	58.3	150 bp	1.0	76.9
rpoA-rps11_p2	GCACTTCCATTGTGGTCTCT	ATGGCTGTAGACCTCCCAAA	50.0	50.0	57.8	58.3	149 bp	1.0	76.9
rpoA-rps11_p3	ATTCGACGCACTTCCATTGT	AATGGCTGTAGACCTCCCAA	45.0	50.0	58.2	58.3	157 bp	1.0	76.8
rpoA-rps11_p4	ATTCGACGCACTTCCATTGT	ATGGCTGTAGACCTCCCAAA	45.0	50.0	58.2	58.3	156 bp	1.0	76.8
rpoA-rps11_p5	GCACTTCCATTGTGGTCTCT	TGGCTGTAGACCTCCCAAAA	50.0	50.0	57.8	58.6	148 bp	1.0	75.1