Hotspot

psbT-psbN

Genome · 108051-108121 · 71 bp

Back to Araucaria

Pi 0.0563

MarkerSeek score 82.00

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: Genome
length_bp: 71
variable_sites: 4
pi: 0.0563
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TTTTCTTCCGAGAACCACCC	CTGGTTATGCCCTGTACACC	50.0	55.0	57.7	58.0	195 bp	1.0	79.2
psbT-psbN_p2	GAACCACCCAAAGTTCCAGA	CTGGTTATGCCCTGTACACC	50.0	55.0	57.6	58.0	184 bp	1.0	78.6
psbT-psbN_p3	GAGAACCACCCAAAGTTCCA	CTGGTTATGCCCTGTACACC	50.0	55.0	57.6	58.0	186 bp	1.0	78.5
psbT-psbN_p4	AGAACCACCCAAAGTTCCAG	CTGGTTATGCCCTGTACACC	50.0	55.0	57.6	58.0	185 bp	1.0	78.5
psbT-psbN_p5	CTTCCGAGAACCACCCAAAG	CTGGTTATGCCCTGTACACC	55.0	55.0	58.5	58.0	191 bp	1.0	77.6