Hotspot

clpP-rrn5

Genome · 105379-105788 · 410 bp

Back to Araucaria

Pi 0.022

MarkerSeek score 67.40

Primer available yes

Label clpP-rrn5

Feature Metadata

label_name: clpP-rrn5
feature_type: igs
region: Genome
length_bp: 410
variable_sites: 9
pi: 0.022
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-rrn5_p1	TGGAACAGGATTACTCACGAG	GCATCGAGCTATTTTTCCGC	47.6	50.0	57.1	58.0	602-603 bp	1.0	66.1
clpP-rrn5_p2	TGGAACAGGATTACTCACGAG	AGTATCGTCACCGCAGTAGA	47.6	50.0	57.1	58.0	568-569 bp	1.0	66.0
clpP-rrn5_p3	TGGAACAGGATTACTCACGAG	TATCGTCACCGCAGTAGAGT	47.6	50.0	57.1	58.0	566-567 bp	1.0	66.0
clpP-rrn5_p4	TGGAACAGGATTACTCACGAG	ACTACCAAGTTCGGGATGGA	47.6	50.0	57.1	58.1	543-544 bp	1.0	66.0
clpP-rrn5_p5	TGGAACAGGATTACTCACGAG	TCCCCTACAGTATCGTCACC	47.6	55.0	57.1	57.9	576-577 bp	1.0	65.8