Hotspot

trnM-CAU-atpB

Genome · 84950-85218 · 269 bp

Back to Araucaria

Pi 0.0336

MarkerSeek score 67.70

Primer available yes

Label trnM-CAU-atpB

Feature Metadata

label_name: trnM-CAU-atpB
feature_type: igs
region: Genome
length_bp: 269
variable_sites: 9
pi: 0.0336
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpB_p1	CTCAGTGGTTAGAGTGTCGC	AGTCTGGCAATAGCAGCATC	55.0	50.0	58.0	58.0	373 bp	1.0	81.7
trnM-CAU-atpB_p2	CTCAGTGGTTAGAGTGTCGC	AAATACGCCCTGCATTCCTT	55.0	45.0	58.0	57.9	408 bp	1.0	80.7
trnM-CAU-atpB_p3	CTCAGTGGTTAGAGTGTCGC	AATACGCCCTGCATTCCTTT	55.0	45.0	58.0	57.9	407 bp	1.0	80.7
trnM-CAU-atpB_p4	GGCGAGAGTCATTGGTTCAA	AGTCTGGCAATAGCAGCATC	50.0	50.0	58.2	58.0	345 bp	1.0	80.5
trnM-CAU-atpB_p5	CTCAGTGGTTAGAGTGTCGC	TTTGAGAAATACGCCCTGCA	55.0	45.0	58.0	57.8	414 bp	1.0	80.3