Hotspot

trnL-UAA-trnF-GAA

Genome · 78784-79242 · 459 bp

Back to Araucaria

Pi 0.0211
MarkerSeek score 59.00
Primer available yes
Label trnL-UAA-trnF-GAA

Feature Metadata

label_name
trnL-UAA-trnF-GAA
feature_type
igs
region
Genome
length_bp
459
variable_sites
9
pi
0.0211
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnF-GAA_p1 GGGTTCAAGTCCCTCTATTCC GTCTGTATGCCAGGAACCAG 52.4 55.0 57.4 58.0 539-562 bp 1.0 72.6
trnL-UAA-trnF-GAA_p2 GGTTCAAGTCCCTCTATTCCC GTCTGTATGCCAGGAACCAG 52.4 55.0 57.4 58.0 538-561 bp 1.0 72.6
trnL-UAA-trnF-GAA_p3 GGGTTCAAGTCCCTCTATTCC TCTGTATGCCAGGAACCAGA 52.4 50.0 57.4 58.0 538-561 bp 1.0 72.4
trnL-UAA-trnF-GAA_p4 GGTTCAAGTCCCTCTATTCCC TCTGTATGCCAGGAACCAGA 52.4 50.0 57.4 58.0 537-560 bp 1.0 72.4
trnL-UAA-trnF-GAA_p5 GGTTCAAGTCCCTCTATTCCC TGGCACGAGGATTTTCAGTC 52.4 50.0 57.4 58.2 507-530 bp 1.0 71.4