Hotspot

psbM-trnD-GUC

Genome · 59099-61304 · 2206 bp

Back to Araucaria

Pi 0.02

MarkerSeek score 69.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: Genome
length_bp: 2206
variable_sites: 44
pi: 0.02
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TGCAACAGCAATAAGAGCGA	TTAGAGTACCGCCCTGTCAA	45.0	50.0	58.2	58.1	2292-2297 bp	1.0	79.7
psbM-trnD-GUC_p2	TGCAACAGCAATAAGAGCGA	ATTGGTTAGAGTACCGCCCT	45.0	50.0	58.2	58.1	2297-2302 bp	1.0	79.3
psbM-trnD-GUC_p3	GCAACAGCAATAAGAGCGAG	TTAGAGTACCGCCCTGTCAA	50.0	50.0	57.6	58.1	2291-2296 bp	1.0	77.6
psbM-trnD-GUC_p4	GCAACAGCAATAAGAGCGAG	ATTGGTTAGAGTACCGCCCT	50.0	50.0	57.6	58.1	2296-2301 bp	1.0	77.2
psbM-trnD-GUC_p5	TGCAACAGCAATAAGAGCGA	TAGAGTACCGCCCTGTCAAG	45.0	55.0	58.2	58.5	2291-2296 bp	1.0	76.0