Hotspot

psbI-trnS-GCU

Genome · 38353-38443 · 91 bp

Back to Araucaria

Pi 0.044

MarkerSeek score 71.40

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: Genome
length_bp: 91
variable_sites: 4
pi: 0.044
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CGGAATCCTGGACGTAAAGG	TAAAGCGGCGGATTGCTAAT	55.0	45.0	58.1	58.0	183 bp	1.0	80.9
psbI-trnS-GCU_p2	CGGAATCCTGGACGTAAAGG	GGGTTCGAATCCCTCTCTCT	55.0	55.0	58.1	58.2	138 bp	1.0	80.1
psbI-trnS-GCU_p3	CGGAATCCTGGACGTAAAGG	TTCGAATCCCTCTCTCTCCG	55.0	55.0	58.1	58.3	135 bp	1.0	79.3
psbI-trnS-GCU_p4	CTATCGAACGATCCAGGACG	TAAAGCGGCGGATTGCTAAT	55.0	45.0	57.7	58.0	201 bp	1.0	78.7
psbI-trnS-GCU_p5	CGGAATCCTGGACGTAAAGG	ATGGCTGAGTGGACTAAAGC	55.0	50.0	58.1	57.6	197 bp	1.0	77.8