Hotspot

psbK-psbI

Genome · 37446-38241 · 796 bp

Back to Araucaria

Pi 0.0201
MarkerSeek score 64.10
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
Genome
length_bp
796
variable_sites
16
pi
0.0201
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 TGGCAAGCTGCTGTAAGTTT CCTTTACGTCCAGGATTCCG 45.0 55.0 58.3 58.1 930-1010 bp 1.0 78.3
psbK-psbI_p2 TTGGCAAGCTGCTGTAAGTT CCTTTACGTCCAGGATTCCG 45.0 55.0 58.3 58.1 931-1011 bp 1.0 78.3
psbK-psbI_p3 TGCCAGTTATCCCTGTGTTC CCTTTACGTCCAGGATTCCG 50.0 55.0 57.5 58.1 971-1051 bp 1.0 76.8
psbK-psbI_p4 TGGCAAGCTGCTGTAAGTTT CGTCCTGGATCGTTCGATAG 45.0 55.0 58.3 57.7 912-992 bp 1.0 76.3
psbK-psbI_p5 TTGGCAAGCTGCTGTAAGTT CGTCCTGGATCGTTCGATAG 45.0 55.0 58.3 57.7 913-993 bp 1.0 76.3