Hotspot

ycf2-trnI-CAU

Genome · 23873-24701 · 829 bp

Back to Araucaria

Pi 0.0218

MarkerSeek score 60.30

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: Genome
length_bp: 829
variable_sites: 15
pi: 0.0218
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	TCTTGCTTTTCAGTATCCAA	TGACCATTCAGCCATGGATG	35.0	50.0	52.4	57.9	765-884 bp	1.0	45.5
ycf2-trnI-CAU_p2	TTCTTGCTTTTCAGTATCCA	TGACCATTCAGCCATGGATG	35.0	50.0	52.4	57.9	766-885 bp	1.0	45.5
ycf2-trnI-CAU_p3	TCTTGCTTTTCAGTATCCAA	CCGCGACTTCCCAATTATGA	35.0	50.0	52.4	58.1	796-915 bp	1.0	45.4
ycf2-trnI-CAU_p4	TTCTTGCTTTTCAGTATCCA	CCGCGACTTCCCAATTATGA	35.0	50.0	52.4	58.1	797-916 bp	1.0	45.4
ycf2-trnI-CAU_p5	TCTTGCTTTTCAGTATCCAAA	CCGCGACTTCCCAATTATGA	33.3	50.0	53.3	58.1	796-915 bp	1.0	45.4