Hotspot

ndhB-trnL-CAA

Genome · 15904-16501 · 598 bp

Back to Araucaria

Pi 0.0225

MarkerSeek score 62.90

Primer available yes

Label ndhB-trnL-CAA

Feature Metadata

label_name: ndhB-trnL-CAA
feature_type: igs
region: Genome
length_bp: 598
variable_sites: 13
pi: 0.0225
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	TGCAATTGCTCAAGAGACCT	GTCTCGCGTGTCTACCATTT	45.0	50.0	57.4	58.0	639-658 bp	1.0	79.4
ndhB-trnL-CAA_p2	TGCAATTGCTCAAGAGACCT	CTCGCGTGTCTACCATTTCA	45.0	50.0	57.4	58.0	637-656 bp	1.0	79.4
ndhB-trnL-CAA_p3	TGCAATTGCTCAAGAGACCT	GCGTGTCTACCATTTCACCA	45.0	50.0	57.4	58.2	634-653 bp	1.0	77.9
ndhB-trnL-CAA_p4	TGCAATTGCTCAAGAGACCT	TCTCGCGTGTCTACCATTTC	45.0	50.0	57.4	57.7	638-657 bp	1.0	77.3
ndhB-trnL-CAA_p5	TGCAATTGCTCAAGAGACCT	TCGCGTGTCTACCATTTCAC	45.0	50.0	57.4	58.3	636-655 bp	1.0	77.1