Hotspot

rrn23-trnA-UGC

Genome · 3617-4546 · 930 bp

Back to Araucaria

Pi 0.0178

MarkerSeek score 61.60

Primer available yes

Label rrn23-trnA-UGC

Feature Metadata

label_name: rrn23-trnA-UGC
feature_type: igs
region: Genome
length_bp: 930
variable_sites: 16
pi: 0.0178
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn23-trnA-UGC_p1	ATTCGGGAATCTCCGGATCT	GACAGTGGTTTACCCTGTGG	50.0	55.0	58.0	58.1	1062-1084 bp	1.0	82.4
rrn23-trnA-UGC_p2	ATTCGGGAATCTCCGGATCT	GGTTCGAGTCCGCTTATCTC	50.0	55.0	58.0	57.9	1031-1053 bp	1.0	82.4
rrn23-trnA-UGC_p3	ATTCGGGAATCTCCGGATCT	GTTCGAGTCCGCTTATCTCC	50.0	55.0	58.0	57.9	1030-1052 bp	1.0	82.4
rrn23-trnA-UGC_p4	GTCTCTGGGTGCCTAGGTAT	GACAGTGGTTTACCCTGTGG	55.0	55.0	57.9	58.1	990-1012 bp	1.0	82.1
rrn23-trnA-UGC_p5	GTCTCTGGGTGCCTAGGTAT	GTTCGAGTCCGCTTATCTCC	55.0	55.0	57.9	57.9	958-980 bp	1.0	82.1