Hotspot

rpl32-trnL-UAG

SSC · 115724-116545 · 822 bp

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Pi 0.0206
MarkerSeek score 70.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
822
variable_sites
64
pi
0.0206
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.97
markerseek_score
70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGTGCGACAAACAAATAAGTCA AGCGTGTCTACCGATTTCAC 36.4 50.0 57.1 58.0 840-891 bp 1.0 66.8
rpl32-trnL-UAG_p2 TGTGCGACAAACAAATAAGTCA CAGCGTGTCTACCGATTTCA 36.4 50.0 57.1 58.0 841-892 bp 1.0 66.8
rpl32-trnL-UAG_p3 TGTGCGACAAACAAATAAGTCA TCCTAAGAGCAGCGTGTCTA 36.4 50.0 57.1 57.9 850-901 bp 1.0 65.8
rpl32-trnL-UAG_p4 TGTGCGACAAACAAATAAGTCA CCTAAGAGCAGCGTGTCTAC 36.4 55.0 57.1 57.8 849-900 bp 1.0 65.2
rpl32-trnL-UAG_p5 TGTGCGACAAACAAATAAGTCA CTAAGAGCAGCGTGTCTACC 36.4 55.0 57.1 57.8 848-899 bp 1.0 65.2