Hotspot

ndhF-rpl32

SSC · 114364-115558 · 1195 bp

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Pi 0.0147
MarkerSeek score 70.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1195
variable_sites
82
pi
0.0147
unique_haplotype_count
19
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTTGCCGTCGGAAAAAGAAG TAAGGCTGCCCAATATCCCT 50.0 50.0 58.0 58.2 1347-1380 bp 1.0 85.8
ndhF-rpl32_p2 TGCCGTCGGAAAAAGAAGAA TAAGGCTGCCCAATATCCCT 45.0 50.0 58.0 58.2 1345-1378 bp 1.0 85.7
ndhF-rpl32_p3 TTGCCGTCGGAAAAAGAAGA TAAGGCTGCCCAATATCCCT 45.0 50.0 58.0 58.2 1346-1379 bp 1.0 85.7
ndhF-rpl32_p4 GTTGCCGTCGGAAAAAGAAG GACAACGCTTTTAAGGCTGC 50.0 50.0 58.0 58.3 1358-1391 bp 1.0 84.5
ndhF-rpl32_p5 TGCCGTCGGAAAAAGAAGAA GACAACGCTTTTAAGGCTGC 45.0 50.0 58.0 58.3 1356-1389 bp 1.0 84.5